Nature Medicine
7, 344 - 349 (2001)
doi:10.1038/85493
MHC-I−restricted presentation of HIV-1 virion antigens without viral replicationFlorence Buseyne1, Sylvie Le Gall2, Claire Boccaccio3, Jean-Pierre Abastado3, Jeffrey D. Lifson4, Larry O. Arthur4, Yves Rivière1, Jean-Michel Heard2
& Olivier Schwartz21
Laboratoire d'Immunopathologie Viralem, Institut Pasteur, Paris, France
2
Unité Rétrovirus et Transfert Génétique, Institut Pasteur, Paris, France
3
ImmunoDesigned Molecules Research Laboratories, Institut des Cordeliers, Paris, France
4
Retroviral Pathogenesis Laboratory, AIDS Vaccine Program, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland, USA
Correspondence should be addressed to Olivier Schwartz schwartz@pasteur.frDendritic cells and macrophages can process extracellular antigens for presentation by MHC-I molecules. This exogenous pathway may have a crucial role in the activation of CD8+ cytotoxic T lymphocytes during human viral infections. We show here that HIV-1 epitopes derived from incoming virions are presented through the exogenous MHC-I pathway in primary human dendritic cells, and to a lower extent in macrophages, leading to cytotoxic T-lymphocyte activation in the absence of viral protein synthesis. Exogenous antigen presentation required adequate virus-receptor interactions and fusion of viral and cellular membranes. These results provide new insights into how anti-HIV cytotoxic T lymphocytes can be activated and have implications for anti-HIV vaccine design.
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