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Nature Medicine 7, 249 - 253 (2001)
doi:10.1038/84708
Multiplex PCR by multicolor fluorimetry and fluorescence melting curve analysis
Kojo S.J. Elenitoba-Johnson1,2, Sandra D. Bohling2, Carl T. Wittwer1,2 & Thomas C. King3
- Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA
- ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA
- Lifespan Medical Center, and Brown University School of Medicine, Providence, Rhode Island, USA
Correspondence to: Kojo S.J. Elenitoba-Johnson1,2 e-mail: kojo.elenitobaj@path.utah.edu
Sequence-specific fluorescent probe-based systems for the detection of nucleic acid sequences include those using linear hybridization probes1, 2, TaqMan probes3, molecular beacons4, 5, 6 and peptide nucleic acid probes7. The linear hybridization probe format involves a fluorescence resonance energy transfer interaction8, 9 between two fluorescently labeled oligonucleotide probes that hybridize adjacent to one another such that one fluorophore functions as the donor, while the other, the acceptor, emits light when juxtaposed to the donor1.
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