 | Figure 3
Nature Medicine
7, 1298 - 1305 (2001)
doi:10.1038/nm1201-1298
Evasion of human innate and acquired immunity by a bacterial homolog of CD11b that inhibits opsonophagocytosisBenfang Lei, Frank R. DeLeo, Nancy P. Hoe, Morag R. Graham, Stacy M. Mackie, Robert L. Cole, Mengyao Liu, Harry R. Hill, Donald E. Low, Michael J. Federle, June R. Scott
& James M. Musser | | | | Figure 3. Mac-dependent inhibition of ROS production during phagocytosis.
a, Mac inhibits ROS production by OPZ-stimulated PMNs. ROS production was measured in unstimulated human PMNs ( ,  ) or those stimulated with OPZ ( , ) in the presence (, ) or absence (, ) of Mac. b, Rate of Mac-dependent inhibition of ROS production by PMNs during phagocytosis. Rates of ROS production were determined for unstimulated PMNs ( ), OPZ-stimulated PMNs () or OPZ-stimulated PMNs incubated with 5.0  g/ml Mac ( ). c and d, Mac inhibits PMN ROS production during receptor-mediated phagocytosis. PMNs were stimulated with C3bi/IgG latex beads (c) or IgG latex beads (d) in the presence () or absence () of Mac (in g/ml). e, Mac inhibits ROS production by PNs stimulated with opsonized GAS. PMNs were stimulated with opsonized GAS serotype M1 strain MGAS 5005 in the presence () or absence () of Mac (in g/ml). c and e show the effect of the MacRGE mutant protein ( ). GAS PGK was used to control for non-specific inhibitory effects in the assay (, in c−e). Insets in a, c, d and e show kinetic plots of ROS production by stimulated PMNs in the absence (black solid line) or presence (blue solid line) of 5.0 g/ml Mac (a, c and e) or indicated concentration (d). Dotted line, fluorescence in unstimulated PMNs. Results for all panels are expressed as the mean  s.d. of 3−6 separate experiments. *, P < 0.01 versus stimulated PMNs in the absence of Mac.
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