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Article
Nature Medicine  6, 278 - 282 (2000)
doi:10.1038/73128

Reversal of insulin-dependent diabetes using islets generated in vitro from pancreatic stem cells

Vijayakumar K. Ramiya1, Michael Maraist1, Karl E. Arfors2, Desmond A. Schatz3, Ammon B. Peck1, 4 & Janet G. Cornelius4

1  Ixion Biotechnology, 13709 Progress Blvd., Box 13, Alachua, Florida 32615, USA

2  Q-Med of Scandinavia, San Diego, California, USA

3  Department of Pediatrics, University of Florida College of Medicine, Gainesville, Florida 32610, USA

4  Department of Pathology, Immunology & Laboratory Medicine, University of Florida College of Medicine, Gainesville , Florida 32610, USA

Correspondence should be addressed to Ammon B. Peck peck.pathology@mail.health.ufl.edu
Ductal structures of the adult pancreas contain stem cells that differentiate into islets of Langerhans. Here, we grew pancreatic ductal epithelial cells isolated from prediabetic adult non-obese diabetic mice in long-term cultures, where they were induced to produce functioning islets containing alpha, beta and delta cells. These in vitro-generated islets showed temporal changes in mRNA transcripts for islet cell-associated differentiation markers, responded in vitro to glucose challenge, and reversed insulin-dependent diabetes after being implanted into diabetic non-obese diabetic mice. The ability to control growth and differentiation of islet stem cells provides an abundant islet source for beta-cell reconstitution in type I diabetes.

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Nature Medicine
ISSN: 1078-8956
EISSN: 1546-170X
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