Nature Medicine
6, 278 - 282 (2000)
doi:10.1038/73128
Reversal of insulin-dependent diabetes using islets generated in vitro
from pancreatic stem cellsVijayakumar K. Ramiya1, Michael Maraist1, Karl E. Arfors2, Desmond A. Schatz3, Ammon B. Peck1, 4
& Janet G. Cornelius41
Ixion Biotechnology, 13709 Progress Blvd.,
Box 13, Alachua, Florida 32615,
USA
2
Q-Med of Scandinavia, San Diego,
California, USA
3
Department of Pediatrics, University of Florida College
of Medicine, Gainesville, Florida 32610,
USA
4
Department of Pathology, Immunology & Laboratory
Medicine, University of Florida College of Medicine, Gainesville
, Florida 32610, USA
Correspondence should be addressed to Ammon B. Peck peck.pathology@mail.health.ufl.eduDuctal structures of the adult pancreas contain stem cells that differentiate
into islets of Langerhans. Here, we grew pancreatic ductal epithelial cells
isolated from prediabetic adult non-obese diabetic mice in long-term cultures,
where they were induced to produce functioning islets containing  , 
and  cells. These in vitro-generated islets showed temporal
changes in mRNA transcripts for islet cell-associated differentiation markers,
responded in vitro to glucose challenge, and reversed insulin-dependent
diabetes after being implanted into diabetic non-obese diabetic mice. The
ability to control growth and differentiation of islet stem cells provides
an abundant islet source for -cell reconstitution in type I diabetes.
|
