Nature Medicine
6, 35 - 40 (2000)
doi:10.1038/71503
A RhoA-derived peptide inhibits syncytium formation induced by respiratory
syncytial virus and parainfluenza virus type 3Manoj K. Pastey1, Tara L. Gower2, Paul W. Spearman2, 3, James E. Crowe Jr.2, 3
& Barney S. Graham1, 21
Department of Medicine, Vanderbilt University School
of Medicine, Nashville, Tennessee 37232, USA
2
Department of Microbiology & Immunology, Vanderbilt
University School of Medicine, Nashville, Tennessee 37232, USA
3
Department of Pediatrics, Vanderbilt University School
of Medicine, Nashville, Tennessee 37232, USA
Correspondence should be addressed to Barney S. Graham Barney.Graham@mcmail.vanderbilt.eduThe fusion glycoproteins of human respiratory syncytial virus (RSV) and
human parainfluenza virus type-3 (PIV-3) mediate virus entry and syncytium
formation. Interaction between the fusion protein of RSV and RhoA, a small
GTPase, facilitates virus-induced syncytium formation. We show here a RhoA-derived
peptide inhibits RSV and syncytium formation induced by RSV and PIV-3, both
in vitro by inhibition of cell-to-cell fusion and in vivo by reduction
of peak titer by 2 log10 in RSV-infected mice. These findings indicate
that the interaction between these two paramyxovirus fusion proteins and RhoA
is an important target for new antiviral strategies.
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