Nature Medicine
6, 29 - 34 (2000)
doi:10.1038/71496
Transplantation of male germ line stem cells restores fertility in infertile
miceTakehiko Ogawa1, 2, Ina Dobrinski1, 3, Mary R. Avarbock1
& Ralph L. Brinster11
Department of Animal Biology, School of Veterinary
Medicine, University of Pennsylvania, 3850 Baltimore Ave., Philadelphia, Pennsylvania 19104, USA
2
T.O. permanent address: Department of Urology, School of Medicine, Yokohama City University, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan
3
I.D. permanent address: Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 W. Street Rd., Kennett Square, Pennsylvania 19348, USA
Correspondence should be addressed to Ralph L. Brinster Azoospermia or oligozoospermia due to disruption of spermatogenesis are
common causes of human male infertility. We used the technique of spermatogonial
transplantation in two infertile mouse strains, Steel (Sl) and dominant
white spotting (W), to determine if stem cells from an infertile male
were capable of generating spermatogenesis. Transplantation of germ cells
from infertile Sl/Sld mutant male mice to infertile
W/Wv or Wv/W54 mutant
male mice restored fertility to the recipient mice. Thus, transplantation
of spermatogonial stem cells from an infertile donor to a permissive testicular
environment can restore fertility and result in progeny with the genetic makeup
of the infertile donor male.
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