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Nature Medicine 5, 843 - 847 (1999)
doi:10.1038/10567

Gene transfer into CD4+ T lymphocytes: Green fluorescent protein-engineered, encephalitogenic T cells illuminate brain autoimmune responses

Alexander Flügel1, Michael Willem2, Tomasz Berkowicz1 & Hartmut Wekerle1

  1. Max-Planck-Institute of Neurobiology, Department of Neuroimmunology, Am Klopferspitz 18A, 82152 Martinsried, Germany.
  2. Max-Planck-Institute of Biochemistry, Martinsried, Germany.

Correspondence to: Hartmut Wekerle1 e-mail: hWekerle@neuro.mpg.de

Encephalitogenic T cells can be isolated readily from naive or antigen-primed immune organs and propagated as clonal populations in vitro1. These T-cell lines have helped us to understand better epitope recognition, TCR gene use, cytokine patterns, and T-cell interactions with components of the CNS (ref.

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