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Nature Medicine 5, 459 - 462 (1999)
doi:10.1038/7458
Enrichment of epithelial cells for molecular studies
Anirban Maitra1,2, Ignacio I. Wistuba1, Arvind K. Virmani1, M. Sakaguchi1, Inwon Park1, Amy Stucky1, Sara Milchgrub2, David Gibbons2, John D. Minna1,3,4 & Adi F. Gazdar1,2
- Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd. , Dallas, Texas 75235, USA
- Department of Pathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas , Texas 75235, USA
- Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75235, USA
- Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas , Texas 75235, USA
Correspondence to: Adi F. Gazdar1,2 e-mail: gazdar@simmons.swmed.edu
Accurate molecular analysis of tumors and their precursor lesions requires the extraction of specific subpopulations of cells (normal, preneoplastic and tumor) from a composite background of multiple cell types. Attempts to obtain pure tumor cell samples have resulted in the genesis of several methods of cell enrichment, including the use of tumor cell lines, xenografted tumors, and microdissection of frozen or paraffin-embedded tissues.
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