Nature Medicine
2, 1038 - 1041 (1996)
doi:10.1038/nm0996-1038
DNA immunization induces protective immunity against B−cell lymphomaAthanasia D. Syrengelas1, 2, Thomas T. Chen1
& Ronald Levy1, 2
1Department of Medicine, Division of Oncology, Stanford University School of Medicine, Stanford University Medical Center, Room M207, Stanford, California 94305-5306, USA
2Correspondence should be addressed to A.D.S. or R.L. Idiotypic determinants of the immunoglobuiin expressed on the surface of B−cell lymphomas are tumor−specific antigens (TSAs), which can be targeted by immunotherapy. Immunization with DNA constructs encoding the idiotype (Id) of a murine B−cell lymphoma induced specific anti−Id antibody responses and protected mice against tumor challenge. Use of DNA encoding an Id/GM−CSF (idiotype/granulocyte−macrophage colony−stimulating factor) fusion protein improved vaccine efficacy, and xenogeneic immunoglobuiin constant region determinants were required for immunogenicity. These results indicate that DNA may be a simple and efficacious means of inducing immune responses against a weak, otherwise unrecognized tumor antigen, provided that additional stimuli are included with the DNA.
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