Nature Medicine
2, 1033 - 1035 (1996)
doi:10.1038/nm0996-1033
Microsatellite alterations in plasma DNA of small cell lung cancer patientsXu Qi Chen1, Maurice Stroun1, Jean-Luc Magnenat2, Laurent P. Nicod2, Anne-Marie Kurt3, Jacqueline Lyautey1, Christine Lederrey1
& Philippe Anker1, 4
1Laboratory of Plant Biochemistry and Physiology, Faculty of Science, Pavilion des Isotopes, 20 boulevard d'Yvoy, Geneva 1211, Switzerland
2Respiratory Division, Department of Medicine, University of Geneva, Geneva 1205, Switzerland
3Department of Pathology, Faculty of Medicine, University of Geneva,Geneva 1205, Switzerland
4Correspondence should be addressed to P.A. Microsatellite instability is an important characteristic of many tumor types1−8 especially those associated with hereditary non−polyposis colorectal carcinoma (HNPCC) syndrome6−8. Microsatellite alterations in 50% of primary small cell lung carcinoma (SCLC) have been found. These alterations were also found in the sputum4. Because neoplastic characteristics such as decreased strand stability9 and ras mutations10−12 have been found in the plasma DNA of cancer patients, we looked for microsatellite alterations in the plasma of SCLC patients. A microsatellite alteration was present in 16 out of 21 (76%) SCLC tumors and in 15 out of 21 (71%) plasma samples. In one case, the alteration was present only in the plasma DNA. If confirmed in larger studies, microsatellite analysis of plasma DNA might constitute a new tool for tumor staging, management and, possibly, detection.
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