Microsatellite alterations in plasma DNA of small cell lung cancer patients
Xu Qi Chen1, Maurice Stroun1, Jean-Luc Magnenat2, Laurent P. Nicod2, Anne-Marie Kurt3, Jacqueline Lyautey1, Christine Lederrey1
& Philippe Anker1, 4
1Laboratory of Plant Biochemistry and Physiology, Faculty of Science, Pavilion des Isotopes, 20 boulevard d'Yvoy, Geneva 1211, Switzerland
2Respiratory Division, Department of Medicine, University of Geneva, Geneva 1205, Switzerland
3Department of Pathology, Faculty of Medicine, University of Geneva,Geneva 1205, Switzerland
4Correspondence should be addressed to P.A.
Microsatellite instability is an important characteristic of many tumor types1−8 especially those associated with hereditary non−polyposis colorectal carcinoma (HNPCC) syndrome6−8. Microsatellite alterations in 50% of primary small cell lung carcinoma (SCLC) have been found. These alterations were also found in the sputum4. Because neoplastic characteristics such as decreased strand stability9 and ras mutations10−12 have been found in the plasma DNA of cancer patients, we looked for microsatellite alterations in the plasma of SCLC patients. A microsatellite alteration was present in 16 out of 21 (76%) SCLC tumors and in 15 out of 21 (71%) plasma samples. In one case, the alteration was present only in the plasma DNA. If confirmed in larger studies, microsatellite analysis of plasma DNA might constitute a new tool for tumor staging, management and, possibly, detection.
REFERENCES
Vogelstein, B. et al. Allelotype of colorectal carcinomas. Science244, 207−211 (1989). | PubMed | ISI | ChemPort |
Thibodeau, S.N., Bren, G. & Schaid, D. Microsatellite instability in cancer of the proximal colon. Science260, 816−819 (1993). | PubMed | ISI | ChemPort |
Merlo, A. et al. Frequent microsatellite instability in primary small cell lung cancer. Cancer Res.34, 2098−2101 (1994).
Mao, L. et al.. . Microsatellite alterations as clonal markers for the detection of human cancer. Proc. Natl. Acad. Sci. USA91, 9871−9875 (1994). | PubMed | ChemPort |
Mao, L. et al.. Molecular detection of primary bladder cancer by microsatellite analysis. Science271, 659−662 (1996). | PubMed | ISI | ChemPort |
Peltomaki, P. et al.. Microsatellite instability is associated with tumors that characterize the hereditary non-polyposis colorectal carcinoma syndrome. Cancer Res. 53, 5853−5855 (1993). | PubMed | ISI | ChemPort |
Lynch, H.T. et al. Genetics, natural history, tumor spectrum and pathology of HNPCC an updated review. Gastroenterology104, 1535−1549 (1993). | PubMed | ISI | ChemPort |
Kolodner, R.D. et al.. Structure of the human MLH1 locus and analysis of a large hereditary nonpolyposis colorectal carcinoma kindred for mlhl mutations. Cancer Res.55, 242−248 (1995). | PubMed | ISI | ChemPort |
Stroun, M. et al.. Neoplastic characteristics of the DNA found in the plasma of cancer patients. Oncology46, 318−322 (1989). | PubMed | ISI | ChemPort |
Vasyukhin, V. et al.. K-ras point mutations in the blood plasma DNA of patients with colorectal tumors, in Challenges of Modern Medicine: Biotechnology Today. (eds. Verna, R. & Shamoo, A.) 141−150 (Ares-Serono Symposia Publications, Rome, 1994).
Vasyukhin, V. et al.. Point mutations of the N-ras in the blood plasma DNA of patients with myelodysplastic syndrome or acute myelogenous leukaemia. Br.H.Haematol.86, 774−779 (1994).
Sorenson, G.D. et al.. Soluble normal and mutated DNA sequences from single-copy genes in human blood. Cancer Epidemiol Biomark. Prev.3, 67−71 (1994) | ISI | ChemPort |
Nagel, S. et al.. Somatic mutations detected by mini- and microsatellite DNA markers reveal clonal intratumor heterogeneity in gastrointestinal cancers. Cancer Res.55, 2866−2870 (1995). | PubMed | ISI | ChemPort |
Nawroz, H., Koch, W., Anker, P., Stroun, M. & Sidransky, D. Microsatellite alterations in serum DNA of head and neck cancer patients. Nature Med.2, 1036−1038 (1996).
