Nature Medicine
2, 1116 - 1121 (1996)
doi:10.1038/nm1096-1116
Oral immunization with an anti−idiotypic antibody to the exoglycolipid antigen protects against experimental Chlamydia trachomatis infectionJudith A. Whittum-Hudson1, 4, Ling-Ling An2, 4, W. Mark Saltzman3, 5, Robert A. Prendergast1
& A. Bruce Macdonald2
1The Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287-9142
2Department of Microbiology, University of Massachusetts, North Pleasant Street, Amherst, Massachusetts 01003, USA
3Department of Chemical Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
4J.A.W.-H. L.-L.A. present address: Department of Neuropharmacology, Scripps Research Institute, 10666 North Toney Pines Road, La folia, California 92034, USA
5W.M.S. present address: School of Chemical Engineering, Cornell University, Campus Road, Ithaca, New York 14853, USA
Chlamydia trachomatis is the leading cause worldwide of preventable infectious blindness (trachoma) and sexually transmitted disease, including nongonoccocal urethritis and pelvic inflammatory disease. To date, no effective vaccine against C. trachomatis infection has been identified. A monoclonal anti−idiotypic antibody (anti−Id) to the chlamydial exoglycolipid antigen (GLXA) was tested in a murine model of ocular chlamydial infection for its ability to induce systemic immunity, which reduces microbiologic and clinical disease. The anti−Id to GLXA, delivered either systemically in soluble form or orally after encapsulation in poly(lactide) microspheres, induced significant protective immunity against ocular challenge of mice with a human biovar of C. trachomatis. Protection was associated with induction of anti−GLXA antibody and anti−chlamydial neutralizing antibody.
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