Figure 6 - MC expression of Ntsr1 is required for optimal MC-dependent reduction of NT-induced hypotension and enhancement of survival after CLP.

(a) PLCs containing 5 10⁴ PMCs from C57BL/6, Ntsr1^-/- or Ntsr2^-/- mice were incubated with NT (10 M) for 30 min at 37 °C. Results are expressed as the percentage of NT remaining after incubation with cells compared to that in samples of NT incubated in vehicle alone at 37 °C (data were pooled from triplicate determinations in the two independent experiments performed, each of which gave similar results). (b,c) Changes in MAP versus baseline levels (baseline = 0, measured 3 min after injection of 300 l saline, intraperitoneal) at various times after injection of NT (6 nmol in 300 l saline, intraperitoneal) (b), and amounts of NT in the peritoneal fluids of these mice at 30 min after NT injection (c), in Kit^W/W-v mice that had been engrafted intraperitoneally with BMCMCs of C57BL/6-Ntsr1^+/+ (n = 7) or -Ntsr1^-/- (n = 6) origin. (d) Survival after moderate CLP (ligation of distal one-half of cecum; one puncture with a 22-gauge needle) in Kit^W/W-v mice that had been engrafted intraperitoneally with BMCMCs of C57BL/6-Ntsr1^+/+ (n = 14) or Ntsr1^-/- (n = 13) origin.