Technical Report abstract
Nature Medicine 14, 454 - 458 (2008)
Published online: 16 March 2008 | Corrected online: 21 March 2008 | doi:10.1038/nm1692
There is an Erratum (May 2008) associated with this Technical Report.
Detection of colonic dysplasia in vivo using a targeted heptapeptide and confocal microendoscopy
Pei-Lin Hsiung1, Jonathan Hardy1, Shai Friedland2,3, Roy Soetikno2,3, Christine B Du1, Amy P Wu1, Peyman Sahbaie2, James M Crawford4, Anson W Lowe3, Christopher H Contag1 & Thomas D Wang2,3
A combination of targeted probes and new imaging technologies provides a powerful set of tools with the potential to improve the early detection of cancer. To develop a probe for detecting colon cancer, we screened phage display peptide libraries against fresh human colonic adenomas for high-affinity ligands with preferential binding to premalignant tissue. We identified a specific heptapeptide sequence, VRPMPLQ, which we synthesized, conjugated with fluorescein and tested in patients undergoing colonoscopy. We imaged topically administered peptide using a fluorescence confocal microendoscope delivered through the instrument channel of a standard colonoscope. In vivo images were acquired at 12 frames per second with 50-
m working distance and 2.5-m (transverse) and 20-m (axial) resolution. The fluorescein-conjugated peptide bound more strongly to dysplastic colonocytes than to adjacent normal cells with 81% sensitivity and 82% specificity. This methodology represents a promising diagnostic imaging approach for the early detection of colorectal cancer and potentially of other epithelial malignancies.
- Department of Pediatrics, Radiology and Microbiology & Immunology, Stanford University School of Medicine, 318 Campus Dr., Rm. E-150, Stanford, California 94305, USA.
- Veterans Affairs Palo Alto Health Care System, 3801 Miranda Ave., Bldg. 100, Palo Alto, California 94304, USA.
- Department of Medicine, Stanford University School of Medicine, 300 Pasteur Dr., Alway Bldg., Rm. M211, Stanford, California 94305, USA.
- Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, P.O. Box 100275, 1600 SW Archer Rd., MSB 649, Gainesville, Florida 32610, USA.
Correspondence to: Thomas D Wang2,3 e-mail: thomaswa@umich.edu
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
RESEARCH
FLAG chemotherapy followed by allogeneic stem cell transplant using nonmyeloablative conditioning induces regression of myelofibrosis with myeloid metaplasiaBone Marrow Transplantation Original Article
Selective expression of gastric mucin MUC6 in colonic sessile serrated adenoma but not in hyperplastic polyp aids in morphological diagnosis of serrated polypsModern Pathology Original Article
See all 15 matches for Research
