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Letter
Nature Medicine 14, 188 - 193 (2008)
Published online: 20 January 2008 | Corrected online: 23 January 2008 | doi:10.1038/nm1706
Dok1 mediates high-fat diet–induced adipocyte hypertrophy and obesity through modulation of PPAR-
phosphorylation
Tetsuya Hosooka1, Tetsuya Noguchi1, Ko Kotani1, Takehiro Nakamura1, Hiroshi Sakaue1, Hiroshi Inoue1,5, Wataru Ogawa1, Kazutoshi Tobimatsu1, Kazuo Takazawa1, Mashito Sakai1, Yasushi Matsuki2, Ryuji Hiramatsu2, Tomoharu Yasuda3,5, Mitchell A Lazar4, Yuji Yamanashi3 & Masato Kasuga1
Abstract
Insulin receptor substrate (IRS)-1 and IRS-2 have dominant roles in the action of insulin1, but other substrates of the insulin receptor kinase, such as Gab1, c-Cbl, SH2-B and APS, are also of physiological relevance2, 3, 4, 5. Although the protein downstream of tyrosine kinases-1 (Dok1) is known to function as a multisite adapter molecule in insulin signaling6, 7, 8, its role in energy homeostasis has remained unclear. Here we show that Dok1 regulates adiposity. Expression of Dok1 in white adipose tissue was markedly increased in mice fed a high-fat diet, whereas adipocytes lacking this adapter were smaller and showed a reduced hypertrophic response to this dietary manipulation. Dok1-deficient mice were leaner and showed improved glucose tolerance and insulin sensitivity compared with wild-type mice. Embryonic fibroblasts from Dok1-deficient mice were impaired in adipogenic differentiation, and this defect was accompanied by an increased activity of the protein kinase ERK and a consequent increase in the phosphorylation of peroxisome proliferator–activated receptor (PPAR)-
on Ser112. Mutation of this negative regulatory site for the transactivation activity of PPAR- blocked development of the lean phenotype caused by Dok1 ablation. These results indicate that Dok1 promotes adipocyte hypertrophy by counteracting the inhibitory effect of ERK on PPAR- and may thus confer predisposition to diet-induced obesity.
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