Letter abstract
Nature Medicine 13, 625 - 630 (2007)
Published online: 8 April 2007 | doi:10.1038/nm1572
Cyclic AMP–regulated exocytosis of Escherichia coli from infected bladder epithelial cells
Brian L Bishop1, Mathew J Duncan1, Jeongmin Song1, Guojie Li2, David Zaas3 & Soman N Abraham1,2,4
The superficial bladder epithelium is a powerful barrier to urine and also serves as a regulator of bladder volume, which is achieved by apical exocytosis of specialized fusiform vesicles during distension of the bladder. We report that type 1 fimbriated uropathogenic Escherichia coli (UPEC) circumvents the bladder barrier by harboring in these Rab27b/CD63-positive and cAMP-regulatable fusiform vesicles within bladder epithelial cells (BECs). Incorporation of UPEC into BEC fusiform compartments enabled bacteria to escape elimination during voiding and to re-emerge in the urine as the bladder distended. Notably, treatment of UPEC-infected mice with a drug that increases intracellular cAMP and induces exocytosis of fusiform vesicles reduced the number of intracellular E. coli.
- Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
- Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA.
- Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA.
- Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Correspondence to: Brian L Bishop1 e-mail: blb7@duke.edu
Correspondence to: Soman N Abraham1,2,4 e-mail: soman.abraham@duke.edu
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