Figure 6 - MIC-1, injected systemically or locally, induces Stat3 phosphorylation in the hypothalamus, even in db/db mice.
From the following article
Tumor-induced anorexia and weight loss are mediated by the TGF-
superfamily cytokine MIC-1
Heiko Johnen, Shu Lin, Tamara Kuffner, David A Brown, Vicky Wang-Wei Tsai, Asne R Bauskin, Liyun Wu, Greg Pankhurst, Lele Jiang, Simon Junankar, Mark Hunter, W Douglas Fairlie, Nicola J Lee, Ronaldo F Enriquez, Paul A Baldock, Eva Corey, Fred S Apple, MaryAnn M Murakami, En-Ju Lin, Chuansong Wang, Matthew J During, Amanda Sainsbury, Herbert Herzog & Samuel N Breit
Nature Medicine 13, 1333 - 1340 (2007) Published online: 4 November 2007
doi:10.1038/nm1677
(a–d) Wild-type BALB/c (a–c) or leptin receptor–deficient db/db (d) mice were injected i.p. with either 100 
g leptin (a), 100 g human MIC-1 (b,d) or control buffer (c). (e–h) Wild-type BALB/c mice also received direct unilateral injection into the hypothalamus of either 1 g of human MIC-1 (e,f), leptin (g) or control buffer (h). Either 20 (e–h) or 30 min (a–d) after injection, mice were perfused, and their brain sections were stained with antibody to P-Stat3. Scale bars: a–d,f–h, 40 m; e, 100 m.
