Loss of the tumor suppressor Vhlh leads to upregulation of Cxcr4 and rapidly progressive glomerulonephritis in mice

Mei Ding, Shiying Cui, Chengjin Li, Serge Jothy, Volker Haase, Brent M Steer, Philip A Marsden, Jeffrey Pippin, Stuart Shankland, Maria Pia Rastaldi, Clemens D Cohen, Matthias Kretzler & Susan E Quaggin

Supplementary Fig. 1 (pdf 72K)
The renal glomerulus.

Supplementary Fig. 2 (pdf 100K)
Generation of transgenic mouse lines.

Supplementary Fig. 3 (pdf 96K)
PCNA staining.

Supplementary Fig. 4 (pdf 168K)
Hif1a protein is increased in podocytes from Vhlh ^loxP/loxP Pod-Cre mice.

Supplementary Fig. 5 (pdf 260K)
Glomerular expression of Hif target genes.

Supplementary Fig. 6 (pdf 120K)
Expression of the Hif target gene Sdf1 in glomeruli of wild-type and mutant mice.

Supplementary Fig. 7 (pdf 52K)
Kaplan-Meier survival curves for mutant Vhlh mice treated with blocking antibody to Cxcr4 or vehicle.

Supplementary Fig. 8 (pdf 160K)
mRNA expression analysis of VHL-HIF pathway molecular targets in glomeruli from patients with pauci-immune RPGN, IgA nephritis or no glomerular disease.

Supplementary Table 1 (pdf 100K)
Gene expression patterns in human RPGN compared with expression in glomeruli from Vhlh ^loxP/loxP Pod-Cre mice.

Supplementary Note (pdf 32K)

Top