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Nature Medicine - 12, 972 - 977 (2006)
Published online: 23 July 2006; | doi:10.1038/nm1371

Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry

Pratip K Chattopadhyay, David A Price, Theresa F Harper, Michael R Betts, Joanne Yu, Emma Gostick, Stephen P Perfetto, Paul Goepfert, Richard A Koup, Stephen C De Rosa, Marcel P Bruchez & Mario Roederer

 
Fig 2 full size
Figure 2. Materials used to manufacture the outer layer of quantum dots have a significant impact on level of non-specific staining.
(a) We titrated streptavidin-conjugated QD655 on human PBMCs pretreated with biotinylated antibody to CD8. Levels of nonspecific staining (that is, 'background', measured in CD8- cells) were substantially higher than the specific CD8+ signal when early quantum dot materials (red lines) were compared to later materials (green lines) that had optimal surface coating including PEG. (b) As a consequence, the resolution of CD8+ and CD8- populations improved markedly with newer quantum dot materials.

 
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