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Article
Nature Medicine 12, 534 - 540 (2006)
Published online: 9 April 2006; | doi:10.1038/nm1392

Forkhead protein FoxO1 mediates Agrp-dependent effects of leptin on food intake

Tadahiro Kitamura, Yun Feng, Yukari Ido Kitamura, Streamson C Chua Jr, Allison W Xu, Gregory S Barsh, Luciano Rossetti & Domenico Accili

Supplementary Fig. 1 (pdf 244K)
FoxO1 localization in Agrp neurons in response to fasting and refeeding.

Supplementary Fig. 2 (pdf 236K)
Survey of FoxO1 localization following adenoviral delivery.

Supplementary Fig. 3 (pdf 52K)
Feeding studies in response to intraperitoneal leptin.

Supplementary Fig. 4 (pdf 56K)
FoxO1-ADA and Delta256 fail to affect Pomc mRNA expression levels.

Supplementary Fig. 5 (pdf 32K)
Western blot analysis of FoxO1 levels in hypothalami from wild-type and Foxo1+/- mice.

Supplementary Fig. 6 (pdf 68K)
Mobility gel-shift assays in the presence of varying amounts of bacterially expressed FoxO1 or Stat3 incubated in vitro with radiolabeled oligonucleotides spanning the FoxO1 and Stat3 binding sites of Agrp or Pomc.

Supplementary Fig. 7 (pdf 56K)
Quantitative analysis of Agrp ChIP assay.


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Nature Medicine
ISSN: 1078-8956
EISSN: 1546-170X
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