 | Figure 4
Nature Medicine 12, 585 - 591 (2006)
Published online: 23 April 2006; | doi:10.1038/nm1398
Endogenous microRNA regulation suppresses transgene expression in hematopoietic lineages and enables stable gene transferBrian D Brown, Mary Anna Venneri, Anna Zingale, Lucia Sergi Sergi
& Luigi Naldini | | | | Figure 4. mir-142-3p effectively segregates gene expression between hematopoietic and nonhematopoietic lineages throughout the organs of transgenic mice.
(a) FACS analysis of GFP expression in the peripheral blood and bone marrow from representative TgN.PGK.GFP.142-3pT (24 copies per genome) and TgN.PGK.GFP (4 copies per genome) transgenic mice. (b) Immunofluorescence of the indicated organs from these mice. GFP (green) was visualized by direct fluorescence. Hematopoietic lineage cells were marked by CD45 immunostaining (red) in all organs analyzed except for the thymus, where CD3 (red) was used to mark thymocytes. In TgN.PGK.GFP mice, pan-cellular GFP expression was detected in the parenchyma and stroma of all organs. Hematopoietic lineage cells appear yellow because of overlap between CD45 staining and GFP expression. In contrast, GFP expression in PGK.GFP.142-3pT transgenic mice was selectively suppressed in the CD45+ Kupffer cells (liver), alveolar (lung) and lamina propria (gut) macrophages, which appear red and are indicated by arrows. In the spleen and thymus, GFP expression was also negative in all hematopoietic lineage cells, despite strong expression within the stroma of these organs. Scale bars, 120  m.
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