Activated protein C inhibits tissue plasminogen activator–induced brain hemorrhage

Tong Cheng, Anthony L Petraglia, Zhang Li, Meenakshisundaram Thiyagarajan, Zhihui Zhong, Zhenhua Wu, Dong Liu, Sanjay B Maggirwar, Rashid Deane, José A Fernández, Barbra LaRue, John H Griffin, Michael Chopp & Berislav V Zlokovic

Supplementary Fig. 1 (pdf 412K)
Immunostaining of collagen IV after transient ischemia in mice determined at 24 h.

Supplementary Fig. 2 (pdf 1M)
Immunostaining of ZO1 and CD-31 after transient ischemia in mice determined at 24 h.

Supplementary Fig. 3 (pdf 264K)
Gelatinolytic activity of recombinant pro-MMP9 and active-MMP-9 on SDS-PAGE.

Supplementary Fig. 4 (pdf 232K)
SDS-PAGE analysis of ¹²⁵I-labeled tPA in the absence and presence of APC (400 nM).

Supplementary Fig. 5 (pdf 284K)
Plasma levels of mouse APC (ng/ml) determined by mouse specific APC ELISA after intravenous administration of mouse APC.

Supplementary Table 1 (pdf 68K)
Physiological parameters before MCAO, during MCAO and 60 min after MCAO.

Supplementary Methods (pdf 116K)

Top