Induction of interleukin-8 preserves the angiogenic response in HIF-1−deficient colon cancer cells

Yusuke Mizukami, Won-Seok Jo, Eva-Maria Duerr, Manish Gala, Jingnan Li, Xiaobo Zhang, Michael A Zimmer, Othon Iliopoulos, Lawrence R Zukerberg, Yutaka Kohgo, Maureen P Lynch, Bo R Rueda & Daniel C Chung

Supplementary Fig. 1 (pdf 812K)
Growth of Caco2^HIF-wt and Caco2^HIF-kd cells in virto (a) and in in vivo as xenografts in nude mice (b).

Supplementary Fig. 2 (pdf 1M)
Immunoblot of HIF-1 and HIF-2 proteins in DLD-1^HIF-WT, DLD-1 ^HIF-kd/1470 and DLD-1 ^HIF-kd/2192 cells in normoxic (N) and hypoxic (H) conditions.

Supplementary Fig. 3 (pdf 1M)
The hypoxic induction of IL-8 in the absence of HIF-1 is observed in many cancer cell lines.

Supplementary Fig. 4 (pdf 1M)
Specificity of the pSR.HIF-11470 and pSR.HIF-12192 constructs was demonstrated by coexpression of HIF-1 expression vectors with synonymous codon mutations that are not affected by the siRNA target sequences.

Supplementary Fig. 5 (pdf 1M)
Oncogenic KRAS plays a role in the hypoxic induction of IL-8 in non-colonic cancers.

Supplementary Table 1 (pdf 40K)
Hypoxic gene expression patterns in DLD-1 cells with or without HIF-1.

Top