Nature Medicine
11, 899 - 904 (2005)
Published online: 19 June 2005; | doi:10.1038/nm1264
A rapid and potent DNA vaccination strategy defined by in vivo monitoring of antigen expressionAdriaan D Bins1, Annelies Jorritsma1, Monika C Wolkers1, Chien-Fu Hung2, T-C Wu2, Ton N M Schumacher1
& John B A G Haanen11
Department of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX
Amsterdam, The Netherlands. 2
Department of Pathology, The Johns Hopkins Medical Institution, The Johns Hopkins University School of Medicine, Ross Research Building, Room 512, 720 Rutland Avenue, Baltimore, Maryland
21205, USA.
Correspondence should be addressed to John B A G Haanen j.haanen@nki.nl Induction of immunity after DNA vaccination is generally considered a slow process. Here we show that DNA delivery to the skin results in a highly transient pulse of antigen expression. Based on this information, we developed a new rapid and potent intradermal DNA vaccination method. By short-interval intradermal DNA delivery, robust T-cell responses, of a magnitude sufficient to reject established subcutaneous tumors, are generated within 12 d. Moreover, this vaccination strategy confers protecting humoral immunity against influenza A infection within 2 weeks after the start of vaccination. The strength and speed of this newly developed strategy will be beneficial in situations in which immunity is required in the shortest possible time.
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