Signal amplification in molecular imaging by pretargeting a multivalent, bispecific antibody

Robert M Sharkey, Thomas M Cardillo, Edmund A Rossi, Chien-Hsing Chang, Habibe Karacay, William J McBride, Hans J Hansen, Ivan D Horak & David M Goldenberg

Supplementary Fig. 1 (pdf 212K)
Quantification of radiotracer uptake during dynamic imaging.

Supplementary Fig. 2 (pdf 148K)
Emailed author 10/5/05

Supplementary Fig. 3 (pdf 96K)
SDS-PAGE analysis of three batches of hBS14 following single-step purification with Affigel-IMP-291 affinity chromatography.

Supplementary Fig. 4 (pdf 32K)
BIAcore sensorgram showing simultaneous HSG and CEA binding for hBS14 multivalent bsMAb, confirming dual specificity of the bsMAb.

Supplementary Fig. 5 (pdf 100K)
^99mTc-IMP-245 bivalent HSG peptid used as the radiotracer for these studies.

Supplementary Table 1 (pdf 16K)
The effect of adjusting the bsMAb doe and interval on the uptake of ^99mTc radiotracer in the tumor and blood.

Supplementary Table 2 (pdf 20K)
Biodistribution of ^99mTc radiotracer in nude mice bearing GW-39 tumors that were given 500 pmol of the di-bsMAb followed 24 h later with 50 pmol (32 Ci) of the ^99mTc radiotracer.

Supplementary Movie 1 (mov 12M)
Dynamic 2-min images showing the uptake and elimination of each ^99mTc agent over 60 min.

Top