Nature Medicine
1, 564 - 569 (1995)
doi:10.1038/nm0695-564
Isolation of novel virus-like sequences associated with human hepatitisJohn N. Simons1, 2, Thomas P. Leary1, George J. Dawson1, Tami J. Pilot-Matias1, A. Scott Muerhoff1, George G. Schlauder1, Suresh M. Desai1
& Isa K. Mushahwar1
1Virus Discovery Group, Experimental Biology Research, Abbott Laboratories, North Chicago, Illinois 60064, USA
2Correspondence should be addressed to J.N.S. Two viruses, GB virus A (GBV-A) and GB virus B (GBV-B), were recently identified in the GB hepatitis agent. Human sera containing antibodies that recognize GBV-A and/or GBV-B recombinant proteins were subjected to polymerase chain reaction studies with degenerate oligonucleotides capable of amplifying a segment of the putative helicase genes from GBV-A, GBV-B or hepatitis C virus. Novel sequences related to members of the Flaviviridae were identified in sera from 12 individuals including 4 individuals with hepatitis. The limited nucleotide sequence identity between GBV-A, GBV-B and HCV sequences suggests that a novel virus, tentatively named GB virus C, may be responsible for some cases of non-A, non-B, non-C, non-D, non-E hepatitis. REFERENCES
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