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Article
Nature Medicine  1, 471 - 477 (1995)
doi:10.1038/nm0595-471

A recombinant Listeria monocytogenes vaccine expressing a model tumour antigen protects mice against lethal tumour cell challenge and causes regression of established tumours

Zhen-Kun Pan1, Georgios Ikonomidis1, Audrey Lazenby2, Drew Pardoll3 & Yvonne Paterson1, 4

  1Department of Microbiology and the University of Pennsylvania Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6076, USA

  2Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA

  3Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA

  4Correspondence should be addressed to Y.P.

Listeria monocytogenes is an intracellular organism that has the unusual ability to live in the cytoplasm of the cell. It is thus a good vector for targeting protein antigens to the cellular arm of the immune response. Here we use a model system, consisting of colon and renal carcinomas that express the influenza virus nucleoprotein and a recombinant L. monocytogenes that secretes this antigen, to test the potential of this organism as a cancer immunotherapeutic agent. We show that this recombinant organism can not only protect mice against lethal challenge with tumour cells that express the antigen, but can also cause regression of established macroscopic tumours in an antigen-specific T-cell-dependent manner.

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ISSN: 1078-8956
EISSN: 1546-170X
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