Nature Medicine
1, 1274 - 1278 (1995)
doi:10.1038/nm1295-1274
Kaposi's sarcoma-associated herpesvirus infects endothelial and spindle cellsChris Boshoff1, Thomas F. Schulz1, 5, Margaret M. Kennedy2, Andrew K. Graham2, Cyril Fisher3, Alero Thomas4, J. O'D. McGee2, Robin A. Weiss1
& John J. O'Leary2, 5
1Virology Laboratory, Institute of Cancer Research, Royal Marsden Hospital, Fulham Road, London SW3 6BJ, UK
2Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital, Oxford OX3 9DU, UK
3Department of Histopathology, Royal Marsden Hospital, Fulham Road, London SW3 6BJ, UK
4The London School of Hygiene and Tropical Medicine, Keppel Street, London WC1, UK
5Correspondence should be addressed to T.F.S. or J.J. O'L. Kaposi's sarcoma (KS), a vascular tumour that contains characteristic spindle cells forming slit-like spaces, may have an infectious aetiology. Recently, sequences of a new human herpesvirus, KSHV/HHV-8, have been identified in both HIV-associated and classical KS. We sought to identify the target cell of this virus in KS tumour tissue. Using PCR in situ hybridization (PCR-ISH) we show that KSHV/HHV-8 is present in the flat endothelial cells lining vascular spaces of KS lesions as well as in typical KS spindle cells. These findings show that KSHV/HHV-8 is present in the cell types thought to represent neoplastic cells in these lesions.
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