Nature Medicine
1, 1029 - 1034 (1995)
doi:10.1038/nm1095-1029
Complete sequencing of the p53 gene provides prognostic information in breast cancer patients, particularly in relation to adjuvant systemic therapy and radiotherapyJonas Bergh1, 7, Torbjörn Norberg2, Sigrid Sjögren1, Anders Lindgren3, 6
& Lars Holmberg4, 5
1Department of Oncology, University of Uppsala, Akademiska sjukhuset, S-751 85 Uppsala, Sweden
2Pharmacia Biotech AB, S-751 82 Uppsala, Sweden
3Department of Pathology, University of Uppsala, Akademiska sjukhuset, S-751 85 Uppsala, Sweden
4Department of Surgery, University of Uppsala, Akademiska sjukhuset, S-751 85 Uppsala, Sweden
5Department of Cancer Epidemiology, University of Uppsala, Akademiska sjukhuset, S-751 85 Uppsala, Sweden
6A.L. present address: Department of Pathology, University Hospital, DK-5000 Odense, Denmark
7Correspondence should be addressed to J.B. The complete coding region of the p53 gene was sequenced from 316 consecutively presented breast cancers, of which 97 were lymph node positive and 206 were node negative. The p53 status was related to prognosis and effect of adjuvant therapy. In all, 69 individual mutations, 29 in node−positive tumours, were demonstrated throughout the whole coding sequence. The mutation sites were partly different for node−positive and node−negative patients. p53 mutations in the evolutionary conserved regions II and V were associated with significantly worse prognosis. Adjuvant systemic therapy, especially with tamoxifen, along with radiotherapy seemed to be of less value to p53 mutation− and lymph node−positive tumours.
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