Nature Medicine
1, 59 - 64 (1995)
doi:10.1038/nm0195-59
HIV-specific cytotoxic T-cells in HIV-exposed but uninfected Gambian womenSarah Rowland-Jones1, 5, Julian Sutton1, Koya Ariyoshi2, Tao Dong1, Frances Gotch1, Steve McAdam1, Denise Whitby3, Sehu Sabally2, Awen Gallimore1, Tumani Corrah2, Masafumi Takiguchi4, Thomas Schultz3, Andre McMichael1
& Hilton Whittle2
1Molecular Immunology Group, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, U.K.
2MRC Laboratories, P.O. Box 273, Fajara, The Gambia.
3Chester Beatty Laboratories, The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
4Department of Tumor Biology, Institute of Medical Science, University of Tokyo, Tokyo 108, Japan.
5Correspondence should be addressed to S.-R.J. A crucial requirement in the rational design of a prophylactic vaccine against the hu-uman immunodeficiency virus (HIV) is to establish whether or not protective immunity can occur following natural infection. The immune response to HIV infection is characterized by very vigorous HIV-specific cytotoxic T-lymphocyte (CTL) activity. We have identified four HIV-1 and HIV-2 cross-reactive peptide epitopes, presented to CTL from HIV-infected Gambians by HLA-B35 (the most common Gambian class I HLA molecule). These peptides were used to elicit HIV-specific CTLs from three out of six repeatedly exposed but HIV-seronegative female prostitutes with HLA-B35. These women remain seronegative with no evidence of HIV infection by polymerase chain reaction or viral culture. Their CTL activity may represent protective immunity against HIV infection.
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