Nature Medicine
1, 53 - 58 (1995)
doi:10.1038/nm0195-53
Basic fibroblast growth factor increases dopaminergic graft survival and function in a rat model of Parkinson's diseaseHldeichi Takayama1, 3, Jasodhara Ray1, Heather K. Raymon1, Andrew Baird2, Joanna Hogg2, Lisa J. Fisher1
& Fred H. Gage1, 4
1Department of Neurosciences, University of California San Digeo, La Jolla, California 92093-0627, USA
2The Whittier Institute, 9894 Genesee Avenue, La jolla, California 92037, USA
3H.T. present address: Department of Neurosurgery, School of Medicine, Keio University, Tokyo, Japan
4Correspondence should be addressed to F.H.G. The clinical use of fetal neural grafts as an intracerebral source of dopamine for patients with Parkinson's disease has met with limited success. Since basic fibroblast growth factor (bFGF) enhances the survival and growth of dopaminergic neurons in vitro, we explored whether cells genetically modified to produce bFGF would improve the functional efficacy of dopaminergic neurons implanted into rats with experimental Parkinson's disease. Results show that bFGF-producing cells grafted together with fetal dopamine neurons have potent growth-promoting effects on the implanted neurons in vivo. Moreover, rats implanted with such co-grafts display the most pronounced behavioural improvements post-grafting. These findings not only provide insight into the function of bFGF in situ, but also suggest an approach for enhancing the survival and function of dopamine neurons grafted into the damaged brain.
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