Article abstract
Nature Immunology 9, 887 - 897 (2008)
Published online: 29 June 2008 | Corrected online: 18 September 2008 | doi:10.1038/ni.1630
There is a Corrigendum (October 2008) associated with this Article.
Differently phosphorylated forms of the cortactin homolog HS1 mediate distinct functions in natural killer cells
Boyd Butler1, Diana H Kastendieck1 & John A Cooper1
Abstract
Here we investigated the involvement of HS1, the hematopoietic cell–specific homolog of cortactin, in the actin-based functions of natural killer cells. Involvement of HS1 in T cell regulation has been established, as HS1 is required for the formation of immune synapses. 'Knockdown' of HS1 in natural killer cells resulted in defective lysis of target cells, cell adhesion, chemotaxis and actin assembly at the lytic synapse. Phosphorylation of the tyrosine residue at position 397 (Tyr397) was required for adhesion to the integrin ligand ICAM-1 and for cytolysis, whereas phosphorylation of Tyr378 was required for chemotaxis. Phosphorylation of Tyr397 was also required for integrin signaling and recruitment of integrins, adaptors and actin to the lytic synapse. Thus, HS1 is essential for signaling and actin assembly in natural killer cells, and the functions of the two phosphorylated tyrosine residues are distinct and separable.
- Department of Cell Biology and Physiology, Washington University, Saint Louis, Missouri 63110, USA.
Correspondence to: Boyd Butler1 e-mail: boyd.butler@wustl.edu
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
NEWS AND VIEWS
Distinct functions for HS1 in chemosensory versus adhesive signalingNature Immunology News and Views (01 Aug 2008)
Talin forges the links between integrins and actinNature Cell Biology News and Views (01 Aug 2003)
See all 3 matches for News And ViewsRESEARCH
Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organsNature Immunology Article (01 Aug 2006)
SKAP-55 regulates integrin adhesion and formation of T cell?APC conjugatesNature Immunology Article (01 Apr 2003)
See all 64 matches for Research
