Figure 2 - Effects of stimulation with Flt3L.

(a) Flow cytometry of spleen and bone marrow cells from mice injected daily for 8 d with PBS (- ; left) or Flt3L (right), gated on CD3^- CD19^- NK1.1^- Ter119^- cells (spleen) or Lin^- (CD3^- CD19^- B220^- NK1.1^- CD11b^- CD11c^- Gr1^- Ter119^- ) Sca-1^- cells (bone marrow). Numbers above plots indicate percent of each myeloid cell type (identified by color in plots). This analysis was done four times. (b) Absolute numbers of cDCs, pDCs, PMNs, red pulp macrophages, monocytes, T cells (Tc) and B cells (Bc) (top) and MDPs in bone marrow (bottom) in response to sustained Flt3L stimulation (8 d). Data are representative of two independent experiments. (c) Developmental potential of Lin^- CSF1R⁺ cells from mice left unstimulated (–) or stimulated for 6 d with Flt3L (+); cDC yield was calculated on the basis of 1 10⁵ injected cells. Data are representative of four independent experiments. (d) Contribution of cells from mice stimulated for 6 d with Flt3L (open bars; CD45.2⁺) and from congenic untreated mice (filled bars; CD45.1⁺) to CD3^- CD19^- B220^- NK1.1^- Ter119^- CD11b^- CD11c^- Gr1^- immature spleen cells (Input) and to spleen cDCs, PMNs and monocytes at 6 d after adoptive transfer of mixed cDC-depleted spleen cell populations to irradiated recipients (CD45.1⁺CD45.2⁺). Data are representative of two independent experiments.