Figure 3 - Activation of NF-
B and MAP kinases in Irak2–/– macrophages in response to MALP-2.
From the following article
Sequential control of Toll-like receptor–dependent responses by IRAK1 and IRAK2
Tatsukata Kawagoe, Shintaro Sato, Kazufumi Matsushita, Hiroki Kato, Kosuke Matsui, Yutaro Kumagai, Tatsuya Saitoh, Taro Kawai, Osamu Takeuchi & Shizuo Akira
Nature Immunology 9, 684 - 691 (2008) Published online: 27 April 2008
doi:10.1038/ni.1606
(a,b) Immunoblot of whole-cell lysates of wild-type and Irak2–/– peritoneal macrophages stimulated for various times (above lanes) with MALP-2, analyzed with antibody to phosphorylated (p-) Erk, p38 or Jnk (a) or anti-I
B
(b). Total Erk, p38 and Jnk serve as a loading control. (c,d) EMSA of NF-B DNA-binding activity in nuclear extracts of wild-type and Irak2–/– macrophages stimulated for various times (above lanes) with MALP-2 (10 ng/ml), assessed with an NF-B-specific probe. Arrows indicate the induced NF-B complex. Results are representative of three independent experiments.
