Supplementary information

From the following article

The sphingosine 1-phosphate receptor 1 causes tissue retention by inhibiting the entry of peripheral tissue T lymphocytes into afferent lymphatics

Levi G Ledgerwood, Girdhari Lal, Nan Zhang, Alexandre Garin, Steven J Esses, Florent Ginhoux, Miriam Merad, Helene Peche, Sergio A Lira, Yaozhong Ding, Yu Yang, Xingxuan He, Edward H Schuchman, Maria L Allende, Jordi C Ochando & Jonathan S Bromberg

Nature Immunology 9, 42 - 53 (2008) Published online: 25 November 2007

doi:10.1038/ni1534

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Supplementary Text and Figures

Supplementary Figures 1–5, Table 2 and Methods

Supplementary Video 1

Time-lapse video of untreated T cells migrating across iSVEC4-10. Colored lines represent paths of individual cells over the course of the experiment.

Supplementary Video 2

Time-lapse video of FTY720-treated T cells migrating across iSVEC4-10. Colored lines represent paths of individual cells over the course of the experiment.

Supplementary Video 3

Three-dimensional rendering of Z-stack images from untreated T cells migrating across an iSVEC4-10 endothelial layer. T cells (green) were stained with CFSE, endothelial cells (red) stained with PKH26, and slides were mounted with DAPI-containing mounting solution (blue).

Supplementary Video 4

Three-dimensional rendering of Z-stack images from FTY720-treated T cells migrating across an iSVEC4-10 endothelial layer. T cells (green) were stained with CFSE, endothelial cells (red) were stained with PKH26, and slides were mounted with DAPI-containing mounting solution (blue).

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