Article abstract


Nature Immunology 8, 753 - 761 (2007)
Published online: 27 May 2007 | doi:10.1038/ni1472

Lymphoid reservoirs of antigen-specific memory T helper cells

Nicolas Fazilleau1,4, Michael D Eisenbraun1,3,4, Laurent Malherbe1, Jessica N Ebright2, Rebecca R Pogue-Caley2,3, Louise J McHeyzer-Williams1 & Michael G McHeyzer-Williams1


How vaccines control the development of antigen-specific effector and memory T helper cells is central to protective immunity but remains poorly understood. Here we found that protein vaccination selected high-affinity, CXCR5+ICOShi follicular B-helper T cells (TFH cells) that developed in draining lymphoid tissue to regulate B cell responses. In the memory phase, reservoirs of antigen-specific CXCR5+ICOSlo TFH cells persisted with less effector activity but accelerated antigen-recall ability. This new compartment of memory TFH cells was retained in draining lymphoid sites with antigen-specific memory B cells, persistent complexes of peptide and major histocompatibility complex class II and continued expression of CD69. Thus, protein vaccination promotes B cell immunity by selecting high-affinity effector TFH cells and creating lymphoid reservoirs of antigen-specific memory TFH cells in vivo.

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  1. Department of Immunology, The Scripps Research Institute, La Jolla, California, USA.
  2. Department of Immunology, Duke University Medical Center, Durham, North Carolina, USA.
  3. Present addresses: MedImmune Vaccines, Mountain View, California 94043, USA (M.D.E.), and INC Research, Raleigh, North Carolina 27609, USA (R.R.P.-C.).
  4. These authors contributed equally to this work.

Correspondence to: Michael G McHeyzer-Williams1 e-mail: mcheyzer@scripps.edu

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