Supplementary information

From the following article

Evolution and diversification of lamprey antigen receptors: evidence for involvement of an AID-APOBEC family cytosine deaminase

Igor B Rogozin, Lakshminarayan M Iyer, Lizhi Liang, Galina V Glazko, Victoria G Liston, Youri I Pavlov, L Aravind & Zeev Pancer

Nature Immunology 8, 647 - 656 (2007) Published online: 29 April 2007

doi:10.1038/ni1463

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Supplementary Fig. 1

Database of translated genomic VLRA cassettes and their sequence logos.

Supplementary Fig. 2

Database of translated genomic VLRB cassettes and their sequence logos.

Supplementary Fig. 3

The sea lamprey VLRA.

Supplementary Fig. 4

Database of 194 mature VLRA sequences aligned to the corresponding genomic cassettes.

Supplementary Fig. 5

Database of 636 mature VLRB sequences aligned to the corresponding genomic cassettes (as in Supplementary Fig. 4).

Supplementary Fig. 6

The sea lamprey cytosine deaminase genes.

Supplementary Fig. 7

Alignment of the AID-APOBEC family with members of the cytosine deaminase superfamily.

Supplementary Fig. 8

In vitro mutagenesis of beta-galactosidase by co-expressed PmCDA1.

Supplementary Table 1

Mutagenic effect of PmCDA1 expression in ung+ or ung- E. coli.

Supplementary Table 2

Spectra of spontaneous and PmCDA1-induced mutations: i) in lacZ in vitro, ii) in E. coli rpoB in vivo, and iii) in S. cerevisiae can1 in vivo.

Supplementary Table 3

Mutagenic effect of PmCDA1 expression in ung+ or ung- S. cerevisiae.

Supplementary Table 4

Induction of intragenic recombination in yeast diploids by PmCDA1.

Supplementary Table 5

Sequence and location of PCR primers.

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