Nature Immunology 7, 827 - 834 (2006)
Published online: 16 July 2006; | doi:10.1038/ni1365
Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organsGlen Pearce1, Veronique Angeli2, Gwendalyn J Randolph2, Tobias Junt3, Ulrich von Andrian3, Hans-Joachim Schnittler4
& Rolf Jessberger1, 21
Institute of Physiological Chemistry, Dresden University of Technology, 01307 Dresden, Germany. 2
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA. 3
Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA. 4
Institute of Physiology, Dresden University of Technology, Dresden 01307, Germany.
Correspondence should be addressed to Rolf Jessberger rolf.jessberger@tu-dresden.de The migration of B cells into secondary lymphoid organs is required for the generation of an effective immune response. Here we analyzed the involvement of SWAP-70, a Rac-interacting protein involved in actin rearrangement, in B cell entry into lymph nodes. We noted reduced migration of Swap70-/- B cells into lymph nodes in vivo. Swap70-/- B cells rolled and adhered, yet accumulated in lymph node high endothelial venules. This defect was not due to impaired integrin expression or chemotaxis. Instead, Swap70-/- B cells aberrantly regulated integrin-mediated adhesion. During attachment, Swap70-/- B cells showed defective polarization and did not form uropods or stabilize lamellipodia at a defined region. Thus, SWAP-70 selectively regulates processes essential for B cell entry into lymph nodes.
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