Nature Immunology 7, 819 - 826 (2006)
Published online: 2 July 2006; | doi:10.1038/ni1358
Foxp1 is an essential transcriptional regulator of B cell developmentHui Hu1, Bin Wang2, 3, Madhuri Borde1, Julie Nardone1, 3, Shan Maika2, Laura Allred2, Philip W Tucker2
& Anjana Rao11
Department of Pathology, Harvard Medical School, CBR Institute for Biomedical Research, Boston, Massachusetts 02115, USA. 2
Department of Molecular Genetics and The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA. 3
Present addresses: Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, USA (B.W.), and Cell Signaling Technology, Danvers, Massachusetts 01923, USA (J.N.).
Correspondence should be addressed to Hui Hu hu@cbr.med.harvard.edu or Anjana Rao arao@cbr.med.harvard.edu Forkhead transcription factors are key participants in development and immune regulation. Here we demonstrate that absence of the gene encoding the forkhead transcription factor Foxp1 resulted in a profound defect in early B cell development. Foxp1 deficiency was associated with decreased expression of all B lineage genes in B220+ fetal liver cells as well as with a block in the transition from pro–B cell to pre–B cell involving diminished expression of recombination-activating genes 1 and 2. Foxp1 bound to the Erag enhancer and was involved in controlling variable-(diversity)-joining recombination of the gene encoding immunoglobulin heavy chain in a B cell lineage–specific way. Our results identify Foxp1 as an essential participant in the transcriptional regulatory network of B lymphopoiesis.
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