Nature Immunology
- 7, 1157 - 1165 (2006)
Published online: 1 October 2006; | doi:10.1038/ni1398
A pathway regulated by cell cycle inhibitor p27Kip1 and checkpoint inhibitor Smad3 is involved in the induction of T cell toleranceLequn Li, Yoshiko Iwamoto, Alla Berezovskaya & Vassiliki A Boussiotis Supplementary Fig. 1 (pdf 28K)
DO11.10 T cells from DO11.10 and DO11.10p27 mice were stimulated with various concentrations of OVA(323–339) peptide in the presence of irradiated antigen presenting cells and proliferation was assessed on d3 of culture. Supplementary Fig. 2 (pdf 28K)
For the intracellular IL-2 staining, CD4+ T cells were harvested from primed or tolerized recipients on day fifteen after immunization and restimulated in vitro with OVA(323–339) in the presence of antigen presenting cells for 24 hours. Supplementary Fig. 3 (pdf 40K)
Downregulation of Smad3 expression by shRNA. Supplementary Fig. 4 (pdf 48K)
Transfection efficiency. Supplementary Fig. 5 (pdf 48K)
DO11.10 and DO11.10p27T cells were adoptively transferred into syngeneic recipients that were subsequently treated with immunizing (priming) stimulus, and cells were then purified and activation of cyclin E was examined as described in Figure 3.
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