 | Figure 1
Nature Immunology
- 7, 1174 - 1181 (2006)
Published online: 8 October 2006; | doi:10.1038/ni1400
Disruption of diacylglycerol metabolism impairs the induction of T cell anergyBenjamin A Olenchock, Rishu Guo, Jeffery H Carpenter, Martha Jordan, Matthew K Topham, Gary A Koretzky & Xiao-Ping Zhong | | | | Figure 1. Costimulation increases the hydrolysis of PtdIns(4,5)P2 and the generation of inositol trisphosphate but does not affect the conversion of DAG to phosphatidic acid.
(a) Identification of 32P-labeled lipids by thin-layer chromatography. Purified, 32P-labeled CD4+ T cells were stimulated (key), then the stimulation was terminated by HCl lysis (time, horizontal axis); 32P-labeled lipids were identified by migration together with PtdIns(4,5)P2 (PIP2) standards. (b) Quantification of inositol trisphosphate (IP3). Unlabeled purified CD4+ T cells were stimulated (key), then stimulation was terminated by perchloric acid lysis (time, horizontal axis). *, P = 0.028 (paired Student's t-test). (c) Identification of 32P-labeled lipids by thin-layer chromatography as described in a; 32P-labeled lipids were identified by migration together with phosphatidic acid (PA) standards.  , antibody to. Data are from four independent experiments (error bars, s.e.m.).
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