Nature Immunology
- 7, 1157 - 1165 (2006)
Published online: 1 October 2006; | doi:10.1038/ni1398
A pathway regulated by cell cycle inhibitor p27Kip1 and checkpoint inhibitor Smad3 is involved in the induction of T cell tolerance
Lequn Li, Yoshiko Iwamoto, Alla Berezovskaya & Vassiliki A Boussiotis
Figure 1.Enhanced proliferation during secondary but not primary responses of naive DO11.10p27 cells to antigen-specific stimulation in vitro. (a,b) Primary responses of DO11.10 and DO11.10p27 T cells stimulated with OVA(323–339) (10 g/ml) in the presence of APCs and analyzed at various times after stimulation for proliferation (a) and IL-2 production (b). (c,d) Secondary responses of DO11.10 and DO11.10p27 cells stimulated for 4 d with OVA(323–339) (1 g/ml) in the presence of irradiated APCs, then collected and restimulated with OVA(323–339) (concentration, horizontal axes) in the presence of irradiated APCs. Proliferation was measured at day 3 after restimulation (c) and IL-2 production was analyzed by ELISA at day 1 after restimulation (d). Results are mean and s.d. of triplicates and are representative of four independent experiments with four mice of each type in each experiment.
cells to antigen-specific stimulation in vitro.
T cells stimulated with OVA(323–339) (10 
g/ml) in the presence of APCs and analyzed at various times after stimulation for proliferation (a) and IL-2 production (b). (c,d) Secondary responses of DO11.10 and DO11.10p27
