Nature Immunology
6, 785 - 792 (2005)
Published online: 26 June 2005; | doi:10.1038/ni1220
Nonstimulatory peptides contribute to antigen-induced CD8−T cell receptor interaction at the immunological synapsePia P Yachi1, Jeanette Ampudia1, Nicholas R J Gascoigne1
& Tomasz Zal1, 21
Department of Immunology, IMM1, The Scripps Research Institute, La Jolla, California
92037, USA. 2
Present address: Department of Immunology, MD Anderson Cancer Center, University of Texas, Houston, Texas
77030, USA.
Correspondence should be addressed to Nicholas R J Gascoigne gascoigne@scripps.edu It is unclear if the interaction between CD8 and the T cell receptor (TCR)−CD3 complex is constitutive or antigen induced. Here, fluorescence resonance energy transfer microscopy between fluorescent chimeras of CD3 and CD8 showed that this interaction was induced by antigen recognition in the immunological synapse. Nonstimulatory endogenous or exogenous peptides presented simultaneously with antigenic peptides increased the CD8-TCR interaction. This finding indicates that the interaction between the intracellular regions of a TCR-CD3 complex recognizing its cognate peptide−major histocompatibility complex (MHC) antigen, and CD8 (plus the kinase Lck), is enhanced by a noncognate CD8-MHC interaction. Thus, the interaction of CD8 with a nonstimulatory peptide-MHC complex helps mediate T cell recognition of antigen, improving the coreceptor function of CD8.
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