Nature Immunology6, 707 - 714 (2005)
Published online: 29 May 2005; | doi:10.1038/ni1210
Stable T cell−dendritic cell interactions precede the development of both tolerance and immunity in vivo
Guy Shakhar1, 4, Randall L Lindquist2, 4, Dimitris Skokos2, Diana Dudziak2, Julie H Huang1, Michel C Nussenzweig2, 3
& Michael L Dustin1
1
Program in Molecular Pathogenesis and Department of Pathology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA.
2
Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA.
3
Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA.
The maturation status of dendritic cells (DCs) determines whether they prime or tolerize T cells. We targeted ovalbumin peptide exclusively to DCs in situ using an antibody to DEC-205 and studied the interaction of DCs with naive CD4+ T cells in tolerizing or priming conditions. We used two-photon microscopy to simultaneously track antigen-specific OT-II T cells, nonspecific T cells and DCs in lymph nodes of living mice. In both tolerance and immunity, OT-II cells arrested on DCs near high endothelial venules beginning shortly after extravasation and regained their baseline speed by 18 h. Thus, early antigen-dependent T cell arrest on DCs is a shared feature of tolerance and priming associated with activation and proliferation.
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