Nature Immunology5, 961 - 967 (2004)
Published online: 8 August 2004; | doi:10.1038/ni1103
RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes
Lisa K Cherry1, 3, Xiaoyu Li2, Pascale Schwab1, Bing Lim2, 3
& Lloyd B Klickstein1
1
Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham & Women's Hospital, Smith Building room 650, 1 Jimmy Fund Way, Boston, Massachusetts 02115, USA.
2
Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, 77 Avenue Louis Pasteur, Boston, Massachusetts 02215, USA.
3
Present addresses: Genzyme Corporation, 1 Mountain Road, Framington, Massachusetts 01701, USA (L.K.C.) and Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672 (B.L.).
Lymphocyte function−associated antigen 1 (LFA-1) is relatively nonadhesive on resting lymphocytes; however, the mechanisms underlying changes in its adhesiveness are poorly understood. In this study, we generated a Jurkat T cell clone, J+hi1.14, that contained low amounts of mRNA for RhoH, a leukocyte-specific inhibitory Rho family member. J+hi1.14 cells expressed constitutively adhesive LFA-1 and the cells bound spontaneously to intracellular adhesion molecules 1, 2 and 3. Reconstitution of RhoH mRNA expression in J+hi1.14 cells reverted the adhesion phenotype to that of wild-type. We obtained similar results using RNA interference in peripheral blood lymphocytes. These data demonstrate that RhoH is required for maintenance of lymphocyte LFA-1 in a nonadhesive state.
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