Our intent in the tutorial is to make the reader familiar with common file formats and with general principles of genomic alignment programs. Both because of space limitations and for clarity, only the UCSC genome browser, the Spidey cDNA alignment tool, and the VISTA and PipMaker genomic comparison tools are described in detail; however, we use many different programs, web-based and locally installed, free and commercial, to create and work with aligned sequences. In addition, new programs are constantly being published and marketed. Below, we have listed some of the, mostly web-based, software that we use.

Resources

Genome browsers

ENSEMBL

NCBI Map Viewer

UCSC Genome Browser


Genomic comparisons (user supplies both sequences)

AVID/VISTA

BLASTZ/PipMaker

Cartwheel

Chaos/Dialign

GS-Aligner (use "services" link)

LAGAN


Genomic comparisons (user-supplied sequence compared to whole genome)

BLAT

BLAST at ENSEMBL

BLAST at NCBI (genomes section)

GenomeVISTA


cDNA to genomic sequence comparisons

est2genome

Spidey


Databases with locus names

DDBJ

ENSEMBL

NCBI Gene Search

Locus Link

SwissProt/TrEMBL


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