Nature Immunology5, 1243 - 1250 (2004)
Published online: 14 November 2004; | doi:10.1038/ni1139
Visualizing dendritic cell networks in vivo
Randall L Lindquist1, 4, Guy Shakhar2, 4, Diana Dudziak1, Hedda Wardemann1, Thomas Eisenreich1, 3, Michael L Dustin2
& Michel C Nussenzweig1, 3
1
Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA.
2
Program in Molecular Pathogenesis and Department of Pathology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA.
3
Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA.
In the steady state, dendritic cells (DCs) in the lymph node induce T cell tolerance to self antigens. Innate signals trigger the maturation of tissue DCs, which migrate into lymph nodes and activate T cells. To examine DCs in vivo, we produced transgenic mice whose DCs expressed enhanced yellow fluorescent protein. Two-photon microscopy of lymph nodes in live mice showed that most of the steady-state DCs were enmeshed in an extensive network and remained in place while actively probing adjacent T cells with their processes. Mature DCs were more motile than steady-state DCs and were rapidly dispersed and integrated into the sessile network, facilitating their interaction with migrating T cells.
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