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Article
Nature Immunology  5, 1235 - 1242 (2004)
Published online: 31 October 2004; | doi:10.1038/ni1134

Distinct T cell dynamics in lymph nodes during the induction of tolerance and immunity

Stéphanie Hugues1, 6, Luc Fetler2, 6, Laura Bonifaz3, 5, Julie Helft4, François Amblard2 & Sebastian Amigorena1

1  Institut National de la Santé et de la Recherche Médicale U365, Immunité et Cancer, Institut Curie, 26 rue d'Ulm, F-75248 Paris Cedex 05, France.

2  Centre National de la Recherche Scientifique UMR 168, Laboratoire de Physico-Chimie Curie, Institut Curie, 26 rue d'Ulm, F-75248 Paris Cedex 05, France.

3  Laboratory of Cellular Physiology and Immunology, Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, New York 10021, USA.

4  Institut National de la Santé et de la Recherche Médicale U520, Biologie Cellulaire de l'Immunité Tumorale, Institut Curie, 26 rue d'Ulm, F-75248 Paris Cedex 05, France.

5  Present address: Research Unit on Autoimmune Diseases, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico.

6  These authors contributed equally to this work.

Correspondence should be addressed to Sebastian Amigorena sebastian.amigorena@curie.fr
Induction of immunity and peripheral tolerance requires contacts between antigen-bearing dendritic cells (DCs) and cognate T cells. Using real-time two-photon microscopy, we have analyzed the dynamics of CD8+ T cells in lymph nodes during the induction of antigen-specific immunity or tolerance. At 15−20 h after the induction of immunity, T cells stopped moving and established prolonged interactions with DCs. In tolerogenic conditions, despite effective initial T cell activation and proliferation, naive T cells remained motile and established serial brief contacts with multiple DCs. Thus, stable DC−T cell interactions occur during the induction of priming, whereas brief contacts may contribute to the induction of T cell tolerance.

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Nature Immunology
ISSN: 1529-2908
EISSN: 1529-2916
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