Nature Immunology
4, 1238 - 1246 (2003)
Published online: 9 November 2003; | doi:10.1038/ni1007
Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutantsDavid Traver, Barry H Paw, Kenneth D Poss, W Todd Penberthy, Shuo Lin
& Leonard I ZonSupplementary Video 1. (mov 1M)
Hematopoietic cell transplantation rescues erythropoiesis and survival of gata-1-/- mutants. Individual gata-1-/- recipient at 8 weeks post-transplantation, showing numbers of donor-derived GFP+ cells similar to those in age-matched GATA-1eGFP donor animals. Supplementary Video 2. (mov 1.8M)
Transplantation of whole kidney marrow from double transgenic donors allows independent visualization of leukocytes (GFP+) and erythrocytes (dsRED+) in translucent recipient embryos. On day 1 post-transplantation, videomicroscopy of tail vessels in gata-1-/- recipient showed slow-moving, round leukocytes, larger leukocytes displaying an end-over-end tumbling migration, and rapidly circulating erythrocytes. Supplementary Video 3. (mov 3M)
Transplantation of DP WKM leads to rapid engraftment of hematopoietic sites in bloodless recipients. Ventral view of a bloodless recipient at 5 days post-transplantation. Robust engraftment of one thymic lobe by GFP+ leukocytes is seen between the eye and the pectoral fin on the right side (top) of the larva. Supplementary Video 4. (mov 2.6M)
bloodless recipients display sustained, multilineage hematopoiesis from donor-derived cells. Robust reconstitution of dsRED+ erythrocytes and GFP+ leukocytes as observed in the tail capillaries of a bloodless recipient at 8 weeks post-transplantation.
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