Nature Immunology
4, 1136 - 1143 (2003)
Published online: 28 September 2003; | doi:10.1038/ni980
Inositol 1,3,4,5-tetrakisphosphate is essential for T lymphocyte developmentValérie Pouillon1, Romana Hascakova-Bartova2, Bernard Pajak3, Emmanuelle Adam4, Françoise Bex5, Valérie Dewaste2, Carine Van Lint4, Oberdan Leo3, Christophe Erneux2
& Stéphane Schurmans11
IRIBHM, IBMM, rue des Professeurs Jeener et Brachet 12, 6041 Gosselies, Belgium. 2
IRIBHM, Campus Erasme, route de Lennik 808, 1070 Brussels, Belgium. 3
Laboratoire de Physiologie Animale, IBMM, rue des Professeurs Jeener et Brachet 12, 6041 Gosselies, Belgium. 4
Laboratoire de Chimie Biologique, IBMM, rue des Professeurs Jeener et Brachet 12, 6041 Gosselies, Belgium. 5
Laboratoire de Microbiologie, CERIA, Avenue E. Gryson 1, 1070 Brussels, Belgium.
Correspondence should be addressed to Stéphane Schurmans sschurma@ulb.ac.beInositol 1,4,5-trisphosphate (Ins(1,4,5)P3) is phosphorylated by Ins(1,4,5)P3 3-kinase, generating inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4). The physiological function of Ins(1,3,4,5)P4 is still unclear, but it has been reported to be a potential modulator of calcium mobilization. Disruption of the gene encoding the ubiquitously expressed Ins(1,4,5)P3 3-kinase isoform B (Itpkb) in mice caused a severe T cell deficiency due to major alterations in thymocyte responsiveness and selection. However, we were unable to detect substantial defects in Ins(1,4,5)P3 amounts or calcium mobilization in Itpkb-/- thymocytes. These data indicate that Itpkb and Ins(1,3,4,5)P4 define an essential signaling pathway for T cell precursor responsiveness and development.
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