Nature Immunology
3, 159 - 168 (2002)
Published online: 7 January 2002; | doi:10.1038/ni753
Role of ICAM-3 in the initial interaction of T lymphocytes and APCsMaría C. Montoya1, David Sancho1, Grégory Bonello2, Yves Collette2, Claire Langlet3, Hai Tao He3, Pedro Aparicio4, Andrés Alcover5, Daniel Olive2
& Francisco Sánchez-Madrid11
Servicio de Inmunología, Hospital de la Princesa, C/ Diego de León 62, Universidad Autónoma de Madrid, 28006 Madrid, Spain. 2
Institut de Cancerologie et d'Immunologie de Marseille, Université de la Mediterranée, Institut Paoli Calmettes, Inserm U119, 27 Boulevard Lei Roure, 13009 Marseille, France. 3
Centre d'Immunologie de Marseille Luminy, Inserm CNRS, Case 906, 13288 Marseille, France. 4
Facultad de Medicina, Universidad de Murcia, Espinardo, 30100 Murcia, Spain. 5
Unité de Biologie des Interactions Cellulaires, CNRS URA 1960, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France.
Correspondence should be addressed to Francisco Sánchez-Madrid fsanchez@hlpr.insalud.esAntigen-independent adhesive interactions between T lymphocytes and antigen-presenting cells (APCs) are essential for scanning for specific antigens on the APC surface and for initiating the immune response. Here we show, through time-lapse imaging of live cells, that the intercellular adhesion molecule 3 (ICAM-3, also known as CD50) is clustered specifically at the region of the T lymphocyte surface that initiates contact with APCs. We describe the role of ICAM-3 in T cell−APC conjugate formation before antigen recognition, in early intracellular signaling and in cytoskeletal rearrangement. Our data indicate that ICAM-3 is important in the initial scanning of the APC surface by T cells and, therefore, in generating the immune response.
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